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KMID : 0861020110260010103
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Korea Journal of Herbology
2011 Volume.26 No. 1 p.103 ~ p.110
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Standardization of Eleutherococcus species and HPLC Method Validation for Quantitative Analysis
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Song Mi-kyoung
Kim Mi-Yeon
Kim Ho-Cheol
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Abstract
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Objective : For the standardization and quality control of eleutheroside E in Eleutherococcus species, HPLC analysis was performed and eleutherosdie E content was compared in 23 kinds of Eleutherococcus species collected from Korea and China.
Methods:The content of eleutheroside E in stem bark of Eleutherococcus species collected from Korea and China were analyzed by HPLC. 0.5% phosphoric acid and acetonitrile was used as mobile solvent. Validation of HPLC analysis method was confirmed by analyzing specificity, linearity, precision and accuracy following ICH guideline.
Results: Content of eleutheroside E was determined to be 1.0-1.6% and 0.5-0.8% in Korean and Chinese E. senticosus, respectively. Content of eleutheroside E in E. sessiliflorus was 0.7-1.1% and 0.2-0.4% respectively in Korean and Chinese origin. All calibration curves showed good linear regression. The method showed good precision and accuracy with intra-day and inter-day variations of 0.880-3.442% (RSD) and 0.606-3.328% (RSD), respectively, and average recovery was of 0.141-1.363% (RSD), for the eleutheroside E analyzed.
Conclusion: These results might be used to establish a criterion of eleutheroside E in Eleutherococcus species.
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KEYWORD
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Eleutherococcus senticosus, Method validation, Standardization, Eleutheroside E, HPLC
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